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Innovative Research成立于1998年,因为我们意识到难以获得可靠,高质量和价格合理的研究材料。 从人类血浆和血清等核心产品开始,Innovative Research已发展成为所有实验室试剂的可靠供应商,包括人类生物制品和ELISA试剂盒。今天,我们生产和供应超过3,000种高品质的人类和动物生物,包括血浆,血清,组织和蛋白质。2007年,我们在密歇根州诺维建造并搬入了我们最先进的设施(如下图所示),以适应我们不断增长的产品线。
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Innovative Research/Mouse tPA Total ELISA Kit/IMTPAKT-TOT/1 Ea

  • 产品编号: IMTPAKT-TOT
  • 美  元  价: $495.00
  • 会  员  价: 待定
  • 品       牌: Innovative Research
  • 产       地: 美国
  • 公       司: Innovative Research
  • 产品分类: ref="." class="index">首页 > Innovative Research/Mouse tPA Total ELISA Kit/IMTPAKT-TOT/1 Ea
  • 公司分类: Assay and ELISA Kits
Size:1 Ea
Innovative Research/Mouse tPA Total ELISA Kit/IMTPAKT-TOT/1 Ea
  • Innovative Research/Mouse tPA Total ELISA Kit/IMTPAKT-TOT/1 Ea
商品介绍

Product Description

Mouse tPA Total ELISA Kit

Mouse Tissue Plasminogen Activator (tPA) Total ELISA Kit is for the sensitive measurement of total mouse tPA antigen is easily performed with this 96 well strip format ELISA kit. Murine tPA binds to the capture antibody coated on the microtiter plate.Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of total mouse tPA antigen in plasma and other BIOLOGical fluid samples is easily performed with this 96 well strip format ELISA kit. The level of tPA antigen in normal plasma was found to be 9.4 ng/ml in NSA/CF-1, 2.4 ng/ml in C57BL6, and 0.4 ng/ml in CD-1 mouse strains. The assay measures total tPA in the 0.1-50 ng/ml range. Samples giving mouse tPA levels above 50 ng/ml should be diluted in blocking buffer before use. Mouse tPA will bind to the capture antibody coated on the microtiter plate.Free and complexed tPA will be detected by the assay.

After appropriate washing steps, anti-mouse tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse tPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

References:

(12-30-09)
Endothelial Progenitor Populations in Differentiating Embryonic Stem Cells. II. Drug Selection and Functional Characterization

S Kim, HA von Recum - Tissue Engineering Part A, 2009 - online.liebertpub.com
...section. Both Mouse tPA Activity Assay (cat. no. IMTPAKT; Innovative Research, Novi, MI) and Mouse tPA Total Antigen Assay (cat. no. IMTPAKT-TOT; Innovative Research) were used according to the manufacturer's protocol. tPA...
品牌介绍

Innovative Research成立于1998年,因为我们意识到难以获得可靠,高质量和价格合理的研究材料。 从人类血浆和血清等核心产品开始,Innovative Research已发展成为所有实验室试剂的可靠供应商,包括人类生物制品和ELISA试剂盒。

今天,我们生产和供应超过3,000种高品质的人类和动物生物,包括血浆,血清,组织和蛋白质。

2007年,我们在密歇根州诺维建造并搬入了我们最先进的设施(如下图所示),以适应我们不断增长的产品线。



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